Acid-cleavable thiomaleamic acid linker for homogeneous antibody–drug conjugation† †Electronic supplementary information (ESI) available: 1H and 13C spectra for all novel compounds, and ES-MS spectra for all reactions with proteins described herein. See DOI: 10.1039/c3cc45220dClick here for additional data file.

Reversible protein affinity-labelling using bromomaleimide-based reagents† †Electronic supplementary information (ESI) available: 1H and 13C spectra for all new and known compounds, and ES-MS spectra for all reactions with proteins described herein. See DOI: 10.1039/c3ob40239hClick here for additional data file.

Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will b...

Copper-catalyzed direct alkylation of heteroarenes† †Electronic supplementary information (ESI) available: Experimental procedures, characterization and copies of 1H and 13C NMR spectra for all new compounds. See DOI: 10.1039/c6sc05622a Click here for additional data file.

Dynamic induction of enantiomeric excess from a prochiral azobenzene dimer under circularly polarized light† †Electronic supplementary information (ESI) available: Synthesis, 1H NMR spectra, 13C NMR spectra, UV absorption plots, HPLC traces, and CD spectra of the compounds; crystallographic information files. CCDC 1003754. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c4sc01993h Click here for additional data file. Click here for additional data file.

A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification† †Electronic supplementary information (ESI) available: LC-MS, ES-MS, deconvoluted spectra and fluorescence emission spectra for all reactions with proteins described herein. Fluorescence emission spectra of superfolder GFP, all the cysteine mutants and their derivatives are given. See DOI: 10.1039/c3sc51333e Click here for additional data file.

Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containi...

Exceptional time response, stability and selectivity in doubly-activated phenyl selenium-based glutathione-selective platform† †Electronic supplementary information (ESI) available: 1H, 13C, 77Se and 2D NMR, HRMS spectra of compounds, details of the synthetic procedure, additional fluorescent spectra, confocal microscopy image, DFT calculation data, live imaging movie. See DOI: 10.1039/c5sc02090e Click here for additional data file. Click here for additional data file.